Preparations

First preparations:

1. Check the de-ionized water bottle, should be filled
2. Check the waste bottle, should be empty
3. Place the reagent bottles (if applicable)
4. Check applicable viscosity of reagents
5. Check the gradient pump tubes by pushing cassettes downwards (no click if correctly fixed and in parallel position)
6. In case of a period of inactivity: Check the functioning of the valves with Hardware check/Lorrca IO/Valves. During toggling ON/OFF each valve. should give a "click". If a pinch valve sticks, perform a "Prime all"

Note: The following steps are applicable for users using software version up to 6.02. Users with software version 6.03 can follow the software instructions.

Start of day rinse:

For Instruments without Oxygenscan and without Luer connection on top of the bob:

1. Prepare 5 ml vial of Elon ISO or Oxy ISO 
2. Select from the menu Deformability Curve 
3. Draw 1 ml Elon or Oxy ISO from the vial with a pipette or pastette and insert this into the cup 
4. Lower the bob and select the Rinse tab 
5. Repeat step 3 and 4 
6. Dry the bob and cup with a tissue 

The instrument is ready to go 

For Instruments with Oxygenscan or with Luer connection on top of the bob: 

1. Prepare 5 ml vial of Elon ISO or Oxy ISO 
2. Select from the menu Deformability Curve 
3. Draw 1,4 ml Elon or Oxy ISO from the vial with a syringe
4. Lower the bob 
5. Empty the syringe through the Luer connection on top of the bob 
6. Select the Rinse tab 
7. Repeat step 3 to 6
8. Dry the bob and cup with a tissue

The instrument is ready to go.

Start each day with performing a control sample (containing blood with normal values) before a sample measurement. This control sample gives reference values for the samples.