Pre-test preparations

Start-up

1. Switch on Laptop/PC and Lorrca.
2. Open the nitrogen cylinder.
3. Lower de Bob into de Cup (to start accommodating to a 37⁰C temperature)
4. Start software
   
   
   Recommendation: Run the 'Self-check' (weekly is set as default):
5. The self-check takes around 10 minutes.
   If the Self Check fails: the pO2 scan will be greyed out in the main menu (screenshot)
   All calibration results are stored in C:Lorrca MaxSis\SelfCheck_V01.txt
   Steps of the Self-check are:
   Display sensor batch number: 200123-001 (example)
   - Step 1: Temperature check: OK (37,0⁰C )
   - Step 2: Flow check: OK
   - Step 3: Deoxygenate check: OK (down to < 0.3 % of ambient saturation level)
   - Step 4: Reoxygenate check: OK (between 97% and 103% of ambient saturation level)
   Verify during the deoxygenation steps (2 and 3) that the N2 flow readout above the bob is at 0.5 liter/minute. Adjust if necessary.
   
6. If the Self-check is canceled, the pO2 sensor is not calibrated, pO2 scans may not be correct.
7. In case the Self-check fails, the reason may be:
   Too cold; the instrument or the DI water may be too cold for a correct verification. If this is the case it will pass the test on the second try.
   Alignment; visually verify that the sensor spot correctly stops at right in front of the fiber-optic.
   (In Hardware check screen, Lorrca IO, set Cup velocity to 1 rps and motor mode to run home)
   Damaged; verify that the sensor spot is not damaged by rubbing off the black silicone backing.
   Expired; the sensor spot may have expired, try a new sensor spot to see whether this one passes the test.
   Run the Self-check again.
8. Perform a 'test run' with any (old) blood sample
9. Perform the cleaning procedure after each run.